Changes in Gene Expression and Morphology from ESC’s to Neurons

Location

Guzman 202, Dominican University of California

Start Date

4-20-2017 1:00 PM

End Date

4-20-2017 1:30 PM

Student Type

Undergraduate - Honors

Faculty Mentor(s)

Kiowa Bower, Ph.D.

Presentation Format

Oral Presentation

Abstract/Description

Embryonic stem cells (ESC’s) are pluripotent cells that can specialize into various cell types. In this study, the pluripotent cells are differentiated into neurons. The ESC’s are capable of undergoing unlimited cell divisions as long as they are sustained under the appropriate conditions. After establishing a stem cell population, the cells were treated with Retinoic Acid to direct the differentiation into neurons. A better understanding of the process of ESC differentiation could give insight to particular areas of therapeutic interest like neurodegenerative disorders such as Huntington’s disease. Our goal is to examine changes in gene expression during the differentiation process with a focus on neurotransmitter receptors by using different drugs. We also tested the effect that exposure to the neurotransmitters dopamine and glutamate had on neuronal differentiation.

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Apr 20th, 1:00 PM Apr 20th, 1:30 PM

Changes in Gene Expression and Morphology from ESC’s to Neurons

Guzman 202, Dominican University of California

Embryonic stem cells (ESC’s) are pluripotent cells that can specialize into various cell types. In this study, the pluripotent cells are differentiated into neurons. The ESC’s are capable of undergoing unlimited cell divisions as long as they are sustained under the appropriate conditions. After establishing a stem cell population, the cells were treated with Retinoic Acid to direct the differentiation into neurons. A better understanding of the process of ESC differentiation could give insight to particular areas of therapeutic interest like neurodegenerative disorders such as Huntington’s disease. Our goal is to examine changes in gene expression during the differentiation process with a focus on neurotransmitter receptors by using different drugs. We also tested the effect that exposure to the neurotransmitters dopamine and glutamate had on neuronal differentiation.