Lack of Artemisinin Resistance in Plasmodium falciparum in Uganda Based on Parasitological and Molecular Assays

Authors

Roland A. Cooper, Department of Natural Sciences and Mathematics, Dominican University of CaliforniaFollow
Melissa D. Conrad, Department of Medicine, University of California, San Francisco, California, USAFollow
Quentin D. Watson, Department of Integrative Biology, University of California, Berkeley
Stephanie J. Huezo, Department of Natural Sciences and Mathematics, Dominican University of CaliforniaFollow
Harriet Ninsiima, Infectious Diseases Research Collaboration, Kampala, Uganda
Patrick Tumwebaze, Infectious Diseases Research Collaboration, Kampala, UgandaFollow
Samuel L. Nsobya, Infectious Diseases Research Collaboration, Kampala, UgandaFollow
Philip J. Rosenthal, Department of Medicine, University of California, San FranciscoFollow

Document Type

Article

Source

Antimicrobial Agents and Chemotherapy

ISSN

0066-4804

Volume

59

Issue

8

First Page

5061

Last Page

5064

Publication Date

8-2015

Department

Natural Sciences and Mathematics

Abstract

We evaluated markers of artemisinin resistance in Plasmodium falciparum isolated in Kampala in 2014. By standard in vitro assays, all isolates were highly sensitive to dihydroartemisinin (DHA). By the ring-stage survival assay, after a 6-h DHA pulse, parasitemia was undetectable in 40 of 43 cultures at 72 h. Two of 53 isolates had nonsynonymous K13-propeller gene polymorphisms but did not have the mutations associated with resistance in Asia. Thus, we did not see evidence for artemisinin resistance in Uganda.

Rights

Copyright © 2015 American Society for Microbiology. All rights reserved.

Publisher Statement

Cooper, R. A., Conrad, M. D., Watson, Q. D., Huezo, S. J., Ninsiima, H., Tumwebaze, P., ... & Rosenthal, P. J. (2015). Lack of artemisinin resistance in Plasmodium falciparum in Uganda based on parasitological and molecular assays. Antimicrobial agents and chemotherapy, AAC-00921.

PubMed ID

26033725