Title

Assessing pressurized liquid extraction for the high-throughput extraction of marine-sponge-derived natural products.

Department

Natural Sciences and Mathematics

Document Type

Article

Source

Journal of Natural Products

Publication Date

3-26-2010

ISSN

1520-6025

Volume

73

Issue

3

First Page

359

Last Page

364

Digital Object Identifier / DOI

https://doi.org/10.1021/np900565a

Abstract

In order to compare the utility of standard solvent partitioning (SSP) versus accelerated solvent extraction (ASE), a series of experiments were performed and evaluated. Overall yields, solvent consumption, processing time, and chemical stability of the fractions obtained by both methods were compared. Five marine sponges were selected for processing and analysis containing 12 structurally distinct, bioactive natural products. Extracts generated using SSP and ASE were assessed for chemical degradation using comparative LC MS-ELSD. The extraction efficiency (EE) of the ASE apparatus was 3 times greater than the SSP method on average, while the total extraction yields (TEY) were roughly equivalent. Furthermore, the ASE methodology required only 2 h to process each sample versus 80 h for SSP, and the LC MS-ELSD from extracts of both methods appeared comparable. These results demonstrate that ASE can serve as an effective high-throughput methodology for extracting marine organisms to streamline the discovery of novel and bioactive natural products.

PubMed ID

20030364

Comments

Dedicated to the late Dr. Richard E. Moore of the University of Hawaii at Manoa for his pioneering work on bioactive natural products.

Rights

Copyright © 2009 The American Chemical Society and American Society of Pharmacognosy

Publisher's Statement

This document is the Accepted Manuscript version of a Published Work that appeared in final form in the Journal of Natural Products, copyright © American Chemical Society after peer review and technical editing by the publisher.

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