Graduation Year

2025

Document Type

Master's Thesis

Degree

Master of Science

Program

Biological Science

Partner Organization

Buck Institute for Research on Aging

Program Director

Patti Culross, MD, MPH

First Reader

Pankaj Kapahi, PhD

Second Reader

Johnathan Rylee, PhD

Abstract

Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by beta-amyloid plaques and tau protein aggregation, leading to neuronal degeneration and memory loss. Affecting over 24 million people worldwide, there is currently no cure, and treatments offer only temporary relief. Sleep disturbances are linked to AD progression, impairing the brain’s clearance of toxic proteins and promoting neuroinflammation. Changes in blood metabolites may provide new therapeutic targets and biomarkers. This study used the Drosophila melanogaster AD “tau” model to test the ability of a candidate metabolite to rescue dementia-associated phenotypes. The metabolite was supplemented in the ad libitum (AL) diet of our diseased fruit fly model to test for improved sleep and amelioration of neurodegenerative phenotypes. Lifespan assays, healthspan assays (such as activity to evaluate neuromuscular health), and sleep assays were employed. The results showed that the metabolite improves sleep by up to 60% and extends lifespan.

Available for download on Wednesday, May 31, 2028

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