Antimicrobial Agents and Chemotherapy
Natural Sciences and Mathematics
We evaluated markers of artemisinin resistance in Plasmodium falciparum isolated in Kampala in 2014. By standard in vitro assays, all isolates were highly sensitive to dihydroartemisinin (DHA). By the ring-stage survival assay, after a 6-h DHA pulse, parasitemia was undetectable in 40 of 43 cultures at 72 h. Two of 53 isolates had nonsynonymous K13-propeller gene polymorphisms but did not have the mutations associated with resistance in Asia. Thus, we did not see evidence for artemisinin resistance in Uganda.
Cooper, Roland A.; Conrad, Melissa D.; Watson, Quentin D.; Huezo, Stephanie J.; Ninsiima, Harriet; Tumwebaze, Patrick; Nsobya, Samuel L.; and Rosenthal, Philip J., "Lack of Artemisinin Resistance in Plasmodium falciparum in Uganda Based on Parasitological and Molecular Assays" (2015). Collected Faculty and Staff Scholarship. 156.
Copyright © 2015 American Society for Microbiology. All rights reserved.
Cooper, R. A., Conrad, M. D., Watson, Q. D., Huezo, S. J., Ninsiima, H., Tumwebaze, P., ... & Rosenthal, P. J. (2015). Lack of artemisinin resistance in Plasmodium falciparum in Uganda based on parasitological and molecular assays. Antimicrobial agents and chemotherapy, AAC-00921.